Silver Phosphate (Ag3PO4) Preparation for Apatite Samples
Preparation of Ag3PO4 in fossil biopatite (after Bassett et al., 2007)
1- Weigh between 1.5 mg and 2 mg of powdered sample in a 2 ml polypropylene vial
with pointed bottom.
2- Add 100 µl of 0.5M HNO3. Use the Vortex Genie so the sample can totally dissolved.
3- Add 75 µl of 0.5M KOH to neutralize the dissolution.
4- Add 200 µl of 0.36M KF so CaF2 can precipitate (along with other fluorides
depending on the apatite composition).
5- Centrifuge the samples during ~ 7 minutes so the solid residue stick to the bottom of
6- Transfer the liquid from the vials to a new vial (don't forget to write the signature on
this new vial!) with a pippete. BE CAREFUL in order to avoid the solid of the bottom!
7- Add 250 µl of silver amine (0.2M AgNO3, 0.35M NH4NO3, 0.74M NH4OH).
8- Leave the vials open in the oven at 50°C overnight. At this point the precipitation of
Ag3PO4 should happen (formation of yellow crystals).
9- In order to recover the Ag3PO4, vials must be centrifuged 5 times during 10 minutes
every time. 1 ml of Milli-Q water must be added in every rinse. Ag3PO4 crystals will
remain stuck to the bottom of the vials. Use the Vortex Genie between every rinse.
10- The remaining liquid is removed and vials are left open drying in the oven at 50°C
11- Place vials in a dessicator and weigh between 200 µg and 300 µg of sample in
pressed silver capsules.
NEWS & EVENTS
UCSC's Stable Isotope Laboratory operations are suspended by order of the Santa Cruz County Health Department
due to the coronavirus pandemic. This directive is in effect until at least May 3rd. The laboratory is still accepting samples during the shutdown and will analyze them in the order received as soon as operations reconvene.
SIL Co-Director Matt McCarthy directed a
weekend program for about 20 Educational Opportunity (EOP) STEM transfer students highlighting the use of stable isotope analyses in phyiscial and biological science inquiry.
UCSC's Stable Isotope Laboratory welcomes new Ocean Sciences faculty member Pratigya Polissar. Dr. Polissar will join the SIL as a co-director
SIL Co-Director Matt McCarthy directed a
weekend program for about 40 Educational Opportunity (EOP) STEM transfer students highlighting the use of stable isotope analyses in phyiscial and biological science inquiry.
SIL Co-director and UCSC Physical and Biological Sciences Dean Paul Koch has been elected 2015 AAAS Fellow for "energetic and innovative leadership in applying stable isotope geochemistry to document and interpret environmental change through the past 65 million years of Earth history.".
Formation of coastal sea ice in North Pacific drives ocean circulation and climate. So indicates evidence published by UCSC scientists Karla Knudson and Christina Ravelo.
Christina Ravelo, Professor of Ocean Sciences at UC Santa Cruz and UCSC Stable Isotope Laboratory co-director, has received the 2013-14 Outstanding
Faculty Award from the Division of Physical and Biological Sciences. The annual award is the division's highest
honor for faculty achievement, recognizing combined excellence in research, teaching, and service.
Long-term human influenced dietary habits of Yosemite National Park black bears explored by UCSC scientist Jack Hopkins, Paul Koch, and colleagues.
SIL Co-Director Paul Koch honored as a Fellow of the California Academy of Sciences. See story here.
Diversity of great white shark diet was shown to be greater than previously thought based on research by UC Santa Cruz colleagues Sora Kim, Paul Koch, and James Estes along with co-author M. Tim Tinker of the U.S. Geological Survey. This work was highlighted in the Los Angeles Times.
SIL Co-Director Paul Koch has been named Dean of the UC Santa Cruz Division of Physical and Biological Sciences.
SIL Co-Director Paul Koch was quoted in the New York Times on research showing that a tiny Paleocene horse Sifrhippus, the first horse, grew even smaller during the Paleocene-Eocene Thermal Maximum climatic warming event.
SIL Co-Director Christina Ravelo has been selected as an American Geophysical Union (AGU) Fellow.
SIL Co-Directors Paul Koch and Jim Zachos's research on Paleocene-Eocene Thermal Maximum (PETM) climatic warming event is featured in an National Geographic article "Earth Before the Ice" in the October 2011 issue.
SIL Co-Director Paul Koch has been appointed Interim Dean of the UC Santa Cruz Division of Physical and Biological Sciences.
SIL co-director Christina Ravelo (Ocean Sciences) sails as co-chief scientist on Integrated Ocean Drilling Project (IODP) Expedition 323 to investigate Bearing Sea Paleoceanography.
SIL co-director Christina Ravelo (Ocean Sciences) gives the 2008 Emiliani Lecture at the American Geophysical Union Meeting in San Francisco on "Lessons from the Pliocene Warm Period and the Onset of Northern Hemisphere Glaciation".
UCSC SIL has been funded by the National Science Foundation for a new Dual-Inlet Isotope Ratio Mass Spectrometer with individual acid drip system for very small calcium carbonate samples.
SIL co-director Jim Zachos (Earth and Planetary Sciences) recieves prestigious Humbolt Research Award. See Humbolt Award for details.
SIL Co-Director Jim Zachos has been selected as an American Geophysical Union (AGU) Fellow.
May 19, 2006: UCSC Stable Isotope Laboratory Symposium
In recognition of the new continuous flow instruments added to the UCSC stable isotope facility a Symposium is being run to highlight the new analytical capabilities.