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University of California
Stable Isotope Laboratory
Earth and Planetary Sciences
1156 High Street Santa Cruz, CA 95064

Lab: (831) 459-5751
Office: (831) 459-5857
Fax: (831) 459-3074


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Elemental Analyzer Run Configuration Tool

Input sample information to assemble EA run datatable.

Average Sample C:N ratio:
Average Sample Weight % Nitrogen or Carbon: %N: %C:
Number of Samples to be Run:
Aveage Mass of Samples to be Run: µg mg
Beginning Autosampler Port:

NOTE: It is very strongly recommended that you consult with UCSC Stable Isotope staff in designing any new EA analysis protocols and run setup. Failure to do so has resulted in poorly designed experiments that degraded study results, reduced sample throughput, and has necessitated some EA users to completely prepare a second batch of samples. It behooves you to tap into our experience.


Row Mass (µg) Type Port Sample Purpose

Sample Type/Mass Guide

Amount of dried
material to be weighed
per sample (mg)
%N
of sample
Amount of N needed
per sample for
isotope analysis (µg)
Possible sample
types
60.000 0.1% 60 soil
12.000 0.5% 60 soil
4.000 to 6.000 1.5% 60 decaying plant litter
3.000 to 4.000 3.0% 60 fresh leaves, algae
2.000 to 3.000 5.0% 60 legumes
0.600 to 1.200 10.0% 60 all animals
0.300 20.0% 60 ammonium salts
0.151 40.0% 60 urea
42-502-360Plant - leaves
36-40% 0.8-1.3% 60 Plant - roots
46-48% 0.4-0.8% 60 Plant - stems
40-44% 0.02-0.06% 60 Plant - wood
43-47% 1.5-3.5% 60 Plant - grain flour (wheat, rye, rice)
45-47% 2.5-5% 60 Plant - grass, alfalfa
0.8-1.2% 0.1-0.15% 60 Soil - low OM
2.5-3.5% 0.2-0.3% 60 Soil - medium OM
10-14% 0.3-1.0% 60 Soil - high OM
0.5-1.5% 0.05-0.15% 60 Sediment - bulk
40% 10.0% 60 Animal, Fish, Invertebrate tissue